After sterilization, allow the jars to completely cool in a clean and sterile setting, such as a laminar flow hood or a glovebox. Once they have cooled, inoculate them with mushroom spawn using sterile methods.
Store the inoculated jars in a warm and dark environment, typically between 18-25°C (64-77°F). The mycelium will begin to colonize the grain substrate, turning it into a suitable medium for mushroom fruiting.
Keep in mind that