7. Cooling and Inoculation: After sterilization, ensure that the jars cool completely in a clean and sterile setting, such as a laminar flow hood or glovebox. Once they have cooled, inoculate them with mushroom spawn using sterile techniques.
8. Incubation and Colonization: Place the inoculated jars in a warm, dark environment, ideally maintained at a temperature of 18-25°C (64-77°F). The mycelium will begin to colonize the grain substrate,