Rephrase this: 7. Cooling and inoculation: After sterilization, allow ars to cool down completely in a clean and sterile environment, such as a laminar flow hood or a glovebox. Once cooled, inoculate with mushroom spawn using sterile techniques. 8. Inc
7. Cooling and Inoculation: After sterilization, ensure that the jars cool completely in a clean and sterile setting, such as a laminar flow hood or glovebox. Once they have cooled, inoculate them with mushroom spawn using sterile techniques.
8. Incubation and Colonization: Place the inoculated jars in a warm, dark environment, ideally maintained at a temperature of 18-25°C (64-77°F). The mycelium will begin to colonize the grain substrate, converting it into a suitable medium for mushroom fruiting.
Note: It's crucial to uphold strict cleanliness and sterility during the grain preparation and sterilization stages to reduce the risk of contamination. Additionally, different mushroom species may have unique requirements for grain preparation and sterilization, so it's wise to refer to species-specific guidelines or seek expert advice.