Rephrase this, personalize it where necessary and make it past tense: 1. Grain selection: Choose a suitable grain such as rye, wheat, or corn (we used guinea corn) as the base material for the substrate. Ensure that the grains are fresh, uncontaminate
1. Grain Selection: I chose a suitable grain, specifically guinea corn, as the base material for the substrate. I made sure that the grains were fresh, uncontaminated, and of high quality.
2. Grain Rinsing and Soaking: I rinsed the grains thoroughly with clean water to eliminate any dust or impurities. I soaked the grains in water for at least 12-24 hours to hydrate and soften them, facilitating better colonization by the mushroom mycelium.
3. Grain Cooking: After soaking, I drained the grains and transferred them to a large cooking pot. I added fresh water to fully cover the grains and brought it to a boil. Then, I reduced the heat to a simmer, cooking the grains for approximately 30-60 minutes until they were adequately softened and fully cooked.
4. Grain Air Drying and Bottling: Once cooked, I drained the grains to remove excess water and allowed them to cool to room temperature. After air-drying for about one to two hours, I loaded the grains into transparent bottles, filling them close to the top. I placed cotton wool on top of each bottle to create a breathable filter for gas exchange during incubation.
5. Sterilization: After bottling, I placed the jars of grains into a pressure pot filled with water and cooked them again for one hour and thirty minutes. Once the time was up, I turned off the gas and stepped away, allowing the pot to cool for several hours before handling. When it was cool, I inspected the bottles in a reasonably clean environment to check for any faults in the bottles or the grains inside them. At this point, I had perfect grains ready for inoculation with mushroom culture.
6. Sterilization Process: The sterilization typically took place for a duration ranging from 15 to 90 minutes at 15-20 psi (pounds per square inch). The high pressure and temperature ensured that all contaminants, including bacteria, molds, and spores, were effectively eliminated.