Last night I activate Discovery plus premium on my BT account, will it effect my eurosport hd channels on the sky q and Signature package.

Activating Discovery+ Premium on your BT account should not affect your Eurosport HD channels on Sky Q or your Signature package. They are generally separate services. However, it's always a good idea to check with BT or your service provider if you have specific concerns, as channel availability and services can vary based on your package and any promotions or changes that might occur.