Reduce the number of words, making it concise yet still stating the valid points: Grain preparation and sterilization are essential steps in mushroom production, as they create an ideal substrate for growth while minimizing contamination risks. For

Grain preparation and sterilization are crucial in mushroom production to ensure optimal growth and minimize contamination. I chose high-quality, uncontaminated guinea corn, rinsed it thoroughly, and cooked it by boiling and simmering for 30 to 60 minutes until softened. After draining and cooling the grains, I air-dried them and placed them in transparent bottles, filling them nearly to the top, covered with cotton wool for gas exchange. I sterilized the jars in a pressure pot for 1.5 hours, ensuring effective elimination of contaminants at 15-20 psi. Once cooled in a sterile environment, I inoculated the jars with mushroom culture using sterile techniques and stored them in a warm, dark place (18-25°C or 64-77°F) for mycelium colonization. Maintaining cleanliness and adhering to species-specific guidelines is essential to reduce contamination risks.